Colorimetric determination of inorganic phosphate in human urine
نویسندگان
چکیده
منابع مشابه
The Determination of Inorganic Phosphate in Urine by Alkalimetric Titration,
In an investigation of the excretion of phosphate and other substances in short periods (chiefly of 1 hour) now being conducted in this laboratory, it has been necessary to make many inorganic phosphate determinations with limited quantities of urine. In most instances the accuracy possible with colorimetric and nephelometric methods has been sufficient, and, after a considerable amount of time...
متن کاملThe Determination of Inorganic Phosphate in Urine by Alkalimetric Titration,
In an investigation of the excretion of phosphate and other substances in short periods (chiefly of 1 hour) now being conducted in this laboratory, it has been necessary to make many inorganic phosphate determinations with limited quantities of urine. In most instances the accuracy possible with colorimetric and nephelometric methods has been sufficient, and, after a considerable amount of time...
متن کاملEnzymatic colorimetric assay of inorganic phosphate.
Vol.33, No.2, 1987 in June 1986, after recahibration of the glucose method, gave results equal to the survey mean: 640 and 1110 mg/L, respectively. The addition of two more calibration points solved our problem. However, we do not want to imply that five or more calibration points are required. The important question is not how many calibration points are used, but rather, are the concentration...
متن کاملA colorimetric determination of inorganic pyrophosphate.
During recent work on the mechanism of the ATP-CoA-acetate1 reaction (1, 2) we observed that, on incubation of yeast enzyme with ATP, CoA, acetate, and hydroxylamine, a substance was formed in the presence of fluoride which, although initially colorless with the Fiske-Subbarow phosphate reagents (3), slowly developed on standing a blue color of a somewhat more purplish shade than that obtained ...
متن کاملEnzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine.
The performance of an enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine is described. Phosphate ions react with inosine in the presence of purine nucleoside phosphorylase to form hypoxanthine; this is oxidized by xanthine oxidase to uric acid with production of hydrogen peroxide. The latter is determined with the aid of the chromogen system peroxidas...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1963
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.12.172